草莓原生质体的培养和植株再生

Protoplast Culture and Plant Regeneration of Strawberry

通过对已分离提纯的草莓原生质体的培养研究 ,比较得出以KM为培养基 ,以 0 .4mol L葡萄糖和 0 .1mol L蔗糖或仅以 0 .5mol L葡萄糖作碳源 ,采用低溶点琼脂糖包埋的培养方式 ,培养密度采用 1× 10 6个 mL ,原生质体出现分裂和小愈伤组织形成的时间都较早。以MS1 、MS2 、MS3 为培养基、采用分步诱导的方法 ,能顺利诱导产生新植株 。

Through the contrast studies of protoplast culture, it was found that the best condition included KM medium 0.4 mol/L glucose and 0.1mol/L sucrose or only 0.5mol/L glucose as carbon hydrate source, the method of LMET embedding. In this condition, both time of the first division of protoplast and microcalli formation were earlier, plant-lets from protoplast-derived callus were regenerated by using MS 1, MS 2 and MS 3 as media and steps differentiation method. It was easy for the plant-lets to root on MS media.