沈阳地区家庭聚集性感染HBV的家庭HBV前C区基因突变率及其临床意义的研究

Pre-Core mutation of HBV among 26 families with history of chronic HBV infection in Shenyang

目的 研究沈阳地区家庭聚集性感染乙型肝炎病毒的家庭HBV前C区 1896位G→A基因突变率及其临床意义。方法 采用PCR RFLP法检测HBV前C区 1896位G→A基因突变。结果 患者及其家庭成员HBV前C区 1896位G→A基因突变发生率分别为 5 6 .3%和 4 0 .5 % ,明显高于患者配偶 2 5 0 %的突变发生率 ,且配偶中抗 HBs阳性率为 2 6 .3%。同时 ,这种突变在慢性乙型肝炎患者中的发生率为 5 2 .4 % ,在HBV携带者中的发生率为 4 4 .4 % ,在慢性重型肝炎患者中的发生率仅为 2 0 0 %。结论 HBV前C区 1896位G→A基因突变的发生 ,可能与HBV的持续感染有关。

Objective To investigate the Pre Core mutations of HBV and clinical significance among families with history of chronic HBV infection. Methods The G to A mutation at nucleotide 1 896 in the pre C gene of HBV DNA was detected by PCR RFLP. Results The results showed that the mutation rate of G to A mutation at nucleotide 1 896 in the pre C gene of HBV DNA was much higher in patients (56.3%) and their family members (40.5%) than in their spouses (25.0%). While the positive rate of anti HBs in their spouses were 26.3%. On the other hand those mutations were much higher in chronic hepatitis B patients (52.4%) and HBV carriers (44.4%) than in chronic severe hepatitis B patients (20.0%). Conclusion The G to A mutation at nucleotide 1 896 in the pre C gene of HBV DNA could be associated with persistent HBV infection.