轮状病毒肠炎患儿血清和大便IL-2,IL-6,IFN-γ动态变化及与临床指标关系的研究

Dynamic variation of serum and stool level of interleukin-2,interleukin-6 and interferon-γ in children with rotavirus enteritis and its relation to clinical manifestations

目的 研究轮状病毒肠炎患儿血清和大便IL 2、IL 6、IFN γ的动态变化 ,及临床指标的关系。方法 采用双抗体夹心ELISA法。结果 119例腹泻患儿RV阳性率 6 3.0 % ,当年 11月份为高峰月。这些细胞因子在病程中呈不同的高峰时相 ,大便IFN γ达峰值最早 ,血清及大便IL 2达峰值最晚 ,大便IL 2与IL 6差异有非常显著性 (P <0 .0 1) ,大便IL 2与IFN γ、IL 6与IFN γ、血清IL 2与IL 6、腹泻恢复时间与大便IL 2、血、便IL 2及IFN γ与淋巴细胞 +单核细胞均差异有非常显著性或差异有显著性 (P <0 .0 1或P <0 .0 5 )。结论 IL 2、IL 6、IFN γ在婴幼儿轮状病毒肠炎中发挥了重要的免疫调控作用 ,且与临床指标密切相关。

Objective This study was conducted,to observe dynamically the serum and stool level of IL 2,IL 6 and IFN γ with RV enteritis,and to analyse the relation of them and their relation to clinical manifestations. Methods The double antibody sandwich ELISA technique was applied.Results The positive rate of RV in 119 enteritis cases was 63.0% and the peak month was Norvember.These cytokines had different peak phases in the course of disease.The earlist cytokine which had peak phase was IFN γ,the last ones were serum and stool IL 2. There was a negitive relation between stool IL 2 and IL 6 ( P <0.01). There were positive relation between IL 2 and IFN γ, stool IL 6 and IFN γ, serum IL 2 and IL 6, stool IL 2 and the recovery time of the enteritis( P <0.01 or P <0.05).Serum and stool level of IL 2 and IFN γ had positive relation to the number of lmphocyte plus mononuclear cells ( P <0.01 or P <0.05).Conclusion IL 2,IL 6 and IFN γ play important roles of immunoregulation in RV enteritis,and have close relation to clinical manifestations.