太阳光及紫外线照射诱发M13mp2噬菌体DNA产生8-OHdG的作用

Formation of 8-hydroxydeoxyguanosine within DNA of M13mp2 Phages Exposed to Sunlight,UVA and UVB

[目的 ]分析太阳光及长波紫外线 (UVA)、中波紫外线 (UVB)诱发Ml3mp2噬菌体DNA产生 8 羟基脱氧鸟苷(8 OHdG)的作用。 [方法 ]用太阳光、UVA和UVB照射处理M13mp2噬菌体样品 ,以HPLC EC检测DNA样品中的 8 OHdG。 [结果 ] 1、3、5h太阳光照射和 2 8、10 8、2 5 0、5 0 0kJ/m2 剂量UVA照射后的样品均可检测到 8 OHdG含量增加 ,并存在与照射剂量的依存关系。较高剂量 (2 6kJ/m2 )UVB照射也可引起 8 OHdG的产生。 [结论 ]太阳光照射致M13mp2噬菌体突变作用中 ,存在DNA的氧化损伤 ,主要是UVA的作用。

To measure 8 hydroxydeoxyguanosine formation in the DNA of M13mp2 phages exposed to sunlight,UVA and UVB. M13mp2 phages were irradiated and the DNA was isolated.8 OHdG was determined using HPLC with electrochemical detector. Exposure to sunlight for 1,3 and 5 h or to UVA at the doses of 28,108,250 or 500 kJ/m 2 induced a dose dependent increase in the level of 8 OHdG. Exposure to UVB at a higher dose (2 6 kJ/m 2) also induced 8 OHdG formation. [Conclusion] The mutagenesis induced by sunlight is related to oxidative damage of DNA and the UVA component might play main role.