应用双抗原夹心ELISA法筛查献血员梅毒螺旋体抗体

ELISA with Double Antigen Sandwich for Screening Speeific Serum Anti-TP Antibody in Blood Donors

目的 :优选一种适宜于献血员梅毒筛查的试验方法。方法 :采用检测梅毒特异性抗体的双抗原夹心ELISA法对献血员进行抗体检测 ,并与RPR检测结果进行比较 ,对两种方法检测结果不一致的标本 ,再用TPHA法进行确证。结果 :ELISA法阳性检出率 0 36 % (41/ 112 71)、RPR法阳性检出率 0 2 6 % (2 9/ 112 71)。ELISA法与TPHA法总符合率 97 5 % (40 / 4 1)、RPR法与TPHA总符合率 6 3 4 1% (2 6 / 4 1)。结论 :ELISA法优于RPR法 ,具有较高的灵敏度和特异性 ,适宜于献血员的筛查 。

Objective To select a sensitive and specific laboratory examination suitable for screening serum anti-TP antibody in blood donors. Methods The serum anti-TP antibody in 11271 blood donors were detected using ELISA with double antigen sandwich and the outcomes were compared wiht those using RPR assay. The conflicting specimen were confinned by repeating the test with TPHA assay. Results The positive rates of serum anti-TP antibody by ELISA with double antigen sandwich and RPR was 0 36%(41/11271) and 0 26%(29/11271),respectivcly. The coincidence of the detecting outcomes by ELISA with double antigen sandwich and RPR with TPHA was 97 5%(40/41) and 63 41%(26/41) respeetively. Conclusion Compared with RPR assay, ELISA with double antigen sandwich has higher sensibility and specificity for sereening serum anti-TP antibody in blood donors.