复方丹参凝胶对感染创面炎症介质及其基因表达的影响

Effect of Compound Danshen Gel on Expression of Inflammation medium in Infected Wound Surface

目的 :观察复方丹参凝胶 (Lipidgelofcompounddanshen ,LGOCDS)对实验性创伤感染创面动物炎症介质及其基因表达的影响。方法 :制作大鼠皮肤切割伤感染模型 ,经复方丹参凝胶治疗后测定 2d、7d、14d局部组织菌量及体内干扰素 (IFN) γ、肿瘤坏死因子 (TNF) α、及其基因表达 (RT PCR、ELISA法 )。结果 :复方丹参凝胶组不同时间IFN γ、TNF α及其基因表达及创面菌量显著低于对照组 (P <0 .0 5 )。结论 :复方丹参凝胶能促进实验性创伤动物感染创面修复 ,并可显著抑制TNF α、IFN

Objective:To Observe the influence of lipid gel of compound Danshen(LGOCDS) on expression of inflammation medium of experimental animals with infected wound surface. Methods: The incised wound models of mouse and with accompanied by infection were replicated. The amount of bacteria in the trauma was measured. Gene expression and protein levels of tumor necrosis factor α(TNF α)and interferon γ(IFN γ)were also detected by RT-PCR and ELISA at 2 d, 7 d, 14 d after treatment of LGOCDS. Results: LGOCDS significantly decreased IFN γ as well as TNF α levels in the lungs. The expressions TNF α?IFN γ and the amount of bacteria in the trauma were lower than that in the control groups at different time. Conclusion: LGOCDS could promote the restoration of the wound surface of the animals with bacterial infection,could decrease the levels of TNF α?IFN γ.