多孔菌漆酶性质研究

Condition and Partial Characterization of Laccase from Polyporus

研究了多孔菌漆酶的产酶曲线及酶作用最适条件;结果表明在该培养条件下,多孔菌第9天达产酶高峰,峰值酶活为412u/ml,酶作用的最适pH值为4.2,最适酶解温度为25℃,Mg2+、Mn2+对漆酶有激活作用,而Ag+、Fe3+和Cl-则能明显抑止漆酶活性.

We studied the laccase of polyporus in the curve of laccase production and optimal condition of laccase reaction. The result showed that polyporus came the laccase production peak which was 412u/mL at the 9th day in this culture condition The optimum temperature and optimum pH of laccase reaction were 25℃ and 4.2.Mg2+and Mn2+increased laccase activity whereas Ag+?Fe3+and Clobviously inhibited it.