急性一氧化碳中毒大鼠脑血液流变学变化及其意义探讨

Hemorheological changes in cerebral circulation of rats with acute carbon monoxide poisoning and its significance

目的 探讨脑循环血液流变学在急性CO中毒后的变化及在急性CO中毒迟发脑病发病中的可能作用。方法 通过腹腔间隔注射CO制备急性CO中毒模型 ,动态检测染毒后大鼠 (即刻组、 1d、 3d、 7d、 14d、 2 1d)颈静脉血的全血粘度 (1s-1、 5s-1、 30s-1、 2 0 0s-1)、血浆粘度、纤维蛋白原 ,并作全血细胞分析。结果 在不同的切变率下(1s-1、 5s-1、 30s-1、 2 0 0s-1) ,染毒即刻组大鼠脑循环全血粘度显著降低 ,染毒后 1～ 14d显著增高 ;染毒后 1、 7、 14d ,大鼠脑循环血浆粘度均显著升高 ;染毒即刻组大鼠红细胞压积显著降低 ,后又逐渐恢复 ,染毒后 7d ,红细胞压积明显高于染毒前 ;染毒后脑循环血浆纤维蛋白原浓度急剧升高 ,14d后逐渐恢复正常 ;染毒即刻组大鼠RBC、Hb、HCV明显降低 ,后即恢复正常。结论 急性CO中毒大鼠恢复期脑血液全血粘度及血浆粘度升高 ,从而增加血流阻力和减慢流速 ,导致脑循环灌注不良 ,发生脑循环障碍。急性CO中毒大鼠早期脑血液全血粘度及红细胞压积、RBC、Hb、HCV明显降低 ,提示CO生理特性可能参与对血液的影响效应。

Objectives To study the hemorheological change after acute carbon monoxide(CO) poisoning and its possible role in etiology of delayed encephalopathy after acute CO poisoning.Methods Animal model of acute CO poisoning was prepared by intraperitoneal injection of CO at regular interval.Whole blood viscosity(with shear rates of 1s -1 ,5s -1 ,30s -1 ,200s -1 ),plasma viscosity,plasma level of fibrinogen and whole blood cell count were measured,for blood samples collected from jugular vein,immediately after exposure to CO,and 1,3,7,14 and 21 days after exposure.Results Whole blood viscosity in cerebral circulation of the rats decreased significantly immediately after exposure to CO with varied shear rates,and increased significantly from 1 day to 14 days after exposure.Plasma viscosity in cerebral circulation of the rats increased significantly 1,7 and 14 days after exposure.Hematocrit(PCV)decreased significantly in the rats immediately after exposure to CO,then gradually restored,and it was significantly higher 7 days after exposure than that before exposure.Plasma level of fibrinogen in cerebral circulation increased abruptly after exposure,and restored to normal 14 days after exposure.Red blood cell count,hemoglobin and hematocrit decreased significantly immediately after exposure,then restored to normal.Conclusions Whole blood viscosity and plasma viscosity in cerebral circulation increased during the recovery stage of acute CO poisoning in rats,causing increase in circulatory resistance and slowdown in blood flow,poor cerebral infusion,and cerebral circulatory disturbance.During the early stage of acute CO poisoning,whole blood viscosity,hematocrit,red blood cell count and hemoglobin decreased significantly,indicating CO with its specific physiological characteristics could be involved in the effects on blood.