慢性镉暴露诱发大鼠肝脏几种酶活性的变化

Changes of Some Liver Enzyme Activities in Rats Induced by Chronic Cadmium-Exposure

选择健康SD雄性成年大鼠60只,随机分成对照组(C组)、镉暴露中剂量组(M组)和高剂量组(H组),每天分别饲喂含镉0、5、10mg/kg饲料的大鼠全价饲料,分别于第3周和第6周检测肝脏中的乳酸脱氢酶(LDH)、γ-谷氨酰转肽酶(GGT)及碱性磷酸酶(AKP).结果显示:在实验过程中,各组间体重、肝脏系数无显著性差异;第3周,与C组相比,只有H组GGT活性显著增强,其余各组酶活性均无显著性差异;第6周,与C组相比,M组AKP活性差异不显著,GGT、LDH活性有显著增强,H组AKP和LDH活性显著增强,GGT活性极显著增强.可见,镉引起肝毒性的机制与酶的活性间存在一定关系.其中GGT是一种敏感性较强的检测镉致肝慢性中毒的生理指标.

Sixty healthy SD male rats were chosen and divided randomly into control group (group C), middle dose group (group M) and high dose group (group H). Group C, M and H were orally dosed daily with 0, 5 and 10?mg with cadmium per kg feed over six weeks. Changes of lactate dehydrogenase (LDH),γglutamyltransferase (GGT) and alkaline phosphatases (AKP) in liver of rats were determined in the 3nd and 6th weeks respectively. The results showed that during the whole experimental period, Body weights and liver to body weight ratios of group C, M and H had no significant differences. In the 3nd week, compared with the enzymes activities of group C, only GGT activities of group H increased prominently. In the 6th week, GGT and LDH activities increased significantly in group M, and so did AKP and LDH activities in group H. At the same time, GGT activities had the most prominently increased in group H. These results suggest cadmium toxicity on rat's liver is associated to activities of enzymes. GGT is a most sensitive biochemical index reflecting cadmium toxicity on liver.