摘要
在太湖猪各类群遗传多样性RAPD分析的基础上,对太湖猪类群间或与其他猪品种间有差异的核DNA标记位点SANDX 1、SANDX 2及SANDX 3进行了克隆,并对SANDX 2、SANDX 3位点的序列进行了测定及e PCR、BLAST分析.
Based on the results of genetic variation analysis of Sus scrofa using RAPD, three anonymous nuclear DNA fragments (loci of SANDX?1, SANDX?2, SANDX?3) were screened for cloning and sequence variability analysis.The sequences of loci SANDX?2 and SANDX?3 were obtained, and were used for e?PCR and BLAST analysis.The results show that the polymorphic molecular markers from anonymous nuclear DNA could be used for genetic analysis of breeds or strains of domestic pigs.Limitations of this study are also discussed.
出处
《南京师大学报(自然科学版)》
CAS
CSCD
2002年第4期95-99,共5页
Journal of Nanjing Normal University(Natural Science Edition)
基金
江苏省教育委员会自然科学基金项目(1996SWX0010S13).