明胶酶A、B在脐血CD_(34)^+细胞及部分白血病细胞系中的表达

Expression of gelatinase A and B in cord blood CD_(34)^+ cells and leukemic cell lines

目的 研究骨髓及脐血CD34+ 细胞及白血病细胞系的明胶酶 ,即基质金属蛋白酶 9(MMP 9,明胶酶B)和基质金属蛋白酶 2 (MMP 2 ,明胶酶A)的产生 ,探讨它们在CD34+ 细胞迁移、归巢中的意义及在白血病发病中的作用。方法 通过MiniMACS磁珠分离技术分离脐血及骨髓的CD34+细胞 ,通过酶谱分析法测定脐血、骨髓CD34 + 细胞及白血病细胞系的无血清条件培养液中明胶酶的表达。结果 在脐血CD34+ 细胞的条件培养液中可测出相对分子质量为 92× 10 3的亮带 ,骨髓CD34+ 细胞的条件培养液中未测到亮带。部分白血病细胞系U937、KG 1a、HL 6 0可产生相对分子质量为 92×10 3和 72× 10 3的亮带 ,J6 1、J6 2细胞系可产生相对分子质量为 92× 10 3的亮带 ,而HEL、Namalva、CEM、K5 6 2、LCL H不产生亮带。结论 脐血CD34+ 细胞可产生MMP 9,而骨髓CD34+ 细胞不产生 ,脐血CD34+ 细胞具有较强的穿透基底膜的迁移能力并与MMP 9相关。在白血病细胞系中 ,部分髓系白血病细胞系产生MMP 9和MMP 2 ,而非髓系白血病细胞系不产生。

Objective To study the expression of gelatinases, including matrix metalloproteinase 9 (MMP 9, gelatinase B) and matrix metalloproteinase 2(MMP 2, gelatinase A), in CD 34 + cells and leukemic cell lines, and explore the significance of gelatinase in migrating and homing capacity of CD 34 + cells, as well as the role of gelatinase in leukemia pathogenesis. Methods CD 34 + cells were isolated from umbilical cord blood and normal bone marrow by Mini MACS system. By zymogram analysis, MMP 2 and MMP 9 were detected in the serum free condition medium of CD 34 + cells and cell lines. Results One brilliant band with molecular weight of 92×10 3 was detected in condition medium of cord blood CD 34 + cells. No band was detected in condition medium of bone marrow CD 34 + cells. Brilliant bands with molecular weight of 92×10 3 and 72×10 3 were detected in the condition medium of U937?KG 1a and HL 60 cell lines, but not in that of HEL?Namalva?CEM?K562 and LCL H cell lines. In the condition media of J6 1 and J6 2 cells only the 92×10 3 band was detected. Conclusions Cord blood CD 34 + cells produced MMP 9, but bone marrow CD 34 + cells did not, partly explains the fact that cord blood CD 34 + cells possessed higher migrating capacity in comparison with bone marrow CD 34 + cells. The expression of MMP 9 and MMP 2 in leukemic cell lines varied.