血管内皮细胞生长因子在乳腺癌中的表达研究

Expression of Vascular Endothelial Growth Factor (VEGF) in Breast

目的 探讨血管内皮细胞生长因子 (Vascularendothelialgrowthfactor ,VEGF)与乳腺癌血管生成及临床病理参数的关系。方法 采用链霉菌抗生物素 -过氧化物酶复合物 (S -P)法 ,检测 4 6例乳腺癌石蜡标本中VEGF的表达及平均微血管密度 (Miscrovesseldensity ,MVD)。结果 VEGF表达定位于肿瘤细胞浆内 ,CD34表达定位于血管内皮细胞浆内。癌组织中VEGF表达阳性率为 76 0 9%。VEGF表达及MVD值与组织学分级(P <0 0 5 )和淋巴结转移密度相关 (P <0 0 1) ,与组织学分型无关 (P >0 0 5 ) ;VEGF阳性组MVD值明显高于VEGF阴性组 (t =2 2 2 0 ,P <0 0 5 )。结论 VEGF与乳腺癌血管生成密切相关 ,并能促进其生长、浸润及转移。检测VEGF和MVD可作为乳腺癌生物学行为的指标之一。

Objective To investigate the relationship of vascular endothelial growth factor (VEGF) and tumor angiogenesis to the clinical pathological parameters of breast carcinoma. Methods S-P immunohistochemical methods were used to detect the expression of VEGF protein and micro-vessel density (MVD) in 46 specimens of breast carcinomas. Results The results showed that VEGF protein located in cytoplasm and CD34 in cytoplasm of vascular endothelial cell. The positive rates of VEGF protein in breast carcinoma were 76.09%, and the expression of CD34 was positive in all 46 samples. Both VEGF expression and MVD value were positively correlated with the histological grade (P<0.05) and lymph node metastasis (P<0.01), but not related to the histological type (P>0.05). The level of MVD was significantly higher in VEGF positive group than in VEGF negative one (t=2.220,P<0.05). Conclusion VEGF might be highly related to angiogenesis of breast carcinoma and promote growth, invasion and metastasis of tumor. The expression of VEGF or MVD might be one of the reference predictors for the biological behavior of breast carcinoma.