P21/WAF1蛋白在肝细胞癌中的表达及DNA含量分析的意义

P21 protein expression and DNA quantitative analysis in human hepatocellular carcinoma

目的 探讨肝细胞癌p2 1/WAF1蛋白的表达情况及DNA含量的定量分析及临床意义。方法 应用免疫组织化学和多功能真彩色图像分析仪检测 60例肝细胞癌 p2 1蛋白表达量和DNA含量 ,同时取 2 0例癌旁组织作对照。结果 60例肝细胞癌 p2 1蛋白阳性表达率为 47% (2 8/60 ) ,癌旁组织为 15 % (3 /2 0 )。 60例肝细胞癌中高分化癌阳性表达率为 13 % (2 /15 ) ,低分化癌为 5 8% (2 6/4 5 )。可见p2 1蛋白在肝细胞癌中的表达明显高于癌旁肝组织 (P <0 .0 5 ) ,低分化癌明显高于高分化癌 (P <0 .0 1)。肝细胞癌DNA相对倍体均值 (U值 )范围为 1.0 8～ 3 .17,U值随病理分级的增高而增高 (P <0 .0 5 )。结论 p2 1蛋白的表达情况与肝细胞癌的发生发展及恶性程度有关 ,肝细胞癌DNA含量与病理学特征及临床生物学行为之间有密切的关系。两者相结合可作为肝细胞癌诊断和预后的参考指标。

Objective To investigate p21 protein expression and DNA content in hepatocelluar carcinoma. Methods p21 protein expression was quantitatively determined by immunohistochemistry in 60 cases of hepatocellular carcinoma. Quantitative analysis of DNA in 60 cases of hepatocellular carcinoma was performed by image cytometry. Simultaneously 20 cases of adjacent tumor tissues were used as contrast.Results The positive rate of p21 protein in 60 cases of hepatocellular carcinoma was 47%(28/60), that in the adjacent tumor tissue was 15%(3/20). The positive rate of p21 protein in the high grade differentiation was 13%(2/15) and in low grade differentiation was 58%(26/45). The p21 proetin expression was significantly higher in tumor than that in adjacent tumor tissue ( P <0.05). Low grade differentiation was significantly higher than high grade differentiation ( P <0.01). The mean relative ploid of DNA (U value) in hepatocellular carcinoma was from 1.08 to 3.17. U value became higher with increase in grading ( P <0.05).Conclusion Abnormal expression of p21 protein is closely correlated with the development and malignant grade of hepatocellular carcinoma. DNA quantity in hepatocellular carcinoma is also closely correlated with the pathological characteristics and its biological behavior. Both of them can serve as indicators of predicting the prognosis and diagnosis hepatocellular carcinoma.