摘要
目的 探讨商陆抗病毒蛋白 (PAP)对HCV的抑制作用。方法 用不同浓度PAP处理HCV感染细胞模型 ,用荧光定量PCR检测HCVRNA。结果 HepG2 细胞内HCV持续表达达 3 0d以上。PAP处理HCV HepG2 细胞后 ,各组细胞内HCVRNA含量在 48h、96h和 14 4h差异非常显著 (P <0 .0 1)。培养上清液HCVRNA含量在 48h时 ,各组间无明显差异 (P >0 .0 5 ) ,但在 96h和 14 4h时 ,各组间差异非常显著 (P <0 .0 1)。此种抑制作用随PAP浓度的增加而增强。结论 PAP具有抑制HCV复制和表达的作用。
Objective To probe the suppressive effect of pokeweed antiviral protein (PAP) on HCV replication. Methods HCV infected cell model was established by using positive HCV RNA serum to infect human liver cancer cell HepG2. Fluorescent quantitative PCR assay was used to detect HCV RNA in infected cells and in culture supernatant after PAP treatment. Results High level of HCV RNA was detected in HCV infected HepG2 cells and in the culture supernatant for over 30 days. The HCV RNA level in cells showed significant difference at 48h, 96h and 144h after PAP treatment in different groups ( F = 46.5 , 35.12 and 26.5 respectively, P < 0.01 ). The difference of HCV RNA level in culture supernatant was not significant at 48h in those groups ( P > 0.05 ), but was significant at 96h and 144h ( F = 18.54 and 7.02 respectively, P < 0.01 ). The suppresive effect increased with increasing PAP concentrations, and was most marked at 10 100μg/ml PAP concentration. The used concentration of PAP did not cause cell death, disturb cell growth and shapes. Conclusion PAP could significantly suppress HCV replication in HCV hepG2 in a dosage dependent manner, and does not have toxic effect on the cultured cell in the concentration.
出处
《肝脏》
2002年第4期233-236,共4页
Chinese Hepatology
基金
湖北省自然基金项目 (2 0 0 1 .ABB .1 4 8)
