腺病毒介导血管内皮生长因子基因转移预防再狭窄的实验研究

The Experimental Study of the Effects of Adenovirus -mediated Vascular Endothelial Growth Factor Gene Transfer on Restenosis

目的 观察腺病毒介导的血管内皮生长因子(VEGF)基因转移对大鼠颈总动脉损伤后新生内膜增生的影响。方法 将颈总动脉球囊损伤的大鼠分为3组,用局部灌注法将50μl溶液注射至颈总动脉。20min后恢复血流。分别于14d和28d处死大鼠。观察VEGF基因转移对再内皮化及内膜增厚的影响。结果 与两对照组比较,动脉损伤后14d和28d时,VEGF基因转移加速血管再内皮化,减少内皮缺失区域(P<0.05);且显著抑制动脉损伤后新生内膜增厚(P<0.01)。结论 重组腺病毒介导VEGF基因转移对血管成形术后再狭窄有一定的预防作用。

Objective To investigate the effects of adenovirus -mediated human vascular en-dothelial growth factor (VEGF) gene transfer on neointimal hyperplasiain in rat carotid artery after balloon injury. Methods After balloon injury, rats were divided into three groups and 50μl solution was instilled into the isolated common carotid segment. The blood was reestablished after 20 min incubation. Rat were killed 14 days or 28 days after gene transfer. The extent of reendothelialization and intimal area to medial area ratio (I/M) were measured among groups. Results Human VEGF165 gene was successfully transducted into the injured carotid segment. VEGF gene transfer accelerated reendothelialization (P all<0. 05)versus two control groups); and decreased the I/M area ratio markedly (P all <0. 01 versus two control groups) at 14 and 28 days after injury. Conclusions The results suggest that recombinant adenovirus-mediated VEGF165 gene transfer may become a potential approach to prevent neointimal hyperplasia after angioplasty.