家兔后肢缺血模型的制作

A Hindlimb Ischemia Model in Rabbits

目的 建立一种肢体缺血的实验动物模型。方法 结扎 2 8只家兔的左后肢的髂动脉远端并切除股总和骨浅动脉导致缺血。定期评估每只家兔缺血的严重程度 ,一直到术后第 90天。结果 血管造影显示术后大腿的细微侧支血管记数率从 0 7± 0 31增加到 2 0± 0 0 2。小腿血压率显著下降 (p <0 0 1)。小腿血流率显著下降(p <0 0 5 )。后肢肌肉的组织学研究显示有萎缩、纤维化和坏死。结论 成功制备家兔持续后肢缺血模型。

Objective To establish an experimental animal model of limb ischemia. The ischemia was persistent and measurable. Methods Ischemia was induced by ligation of the distal iliac artery and excision of the common and superficial femoral arteries in the left hindlimb of 28 rabbits. The severity of the ischemia in each animal was evaluated regularly until postoperative 90 days. Results Angiography demonstrated minimal collaterals number ratio in the thigh was increased from 0.7±0.31 to 2.0±0.02 postoperatively. The calf blood pressure ratio was decreased significantly ( p <0.01). The calf blood flow ratio was decreased remarkably ( p <0.05). Histologic study of the hindlimb muscle showed atrophy, fibrosis and necrosis. Conclusion Persistent hindlimb ischemia model in the rabbit was made successfully.