HPLC测定通窍鼻炎胶囊中欧前胡素的含量

Determination of imperatorin in Tongqiao Biyan capsules by HPLC

目的 建立通窍鼻炎胶囊中欧前胡素的含量测定方法。方法 采用HPLC ,ShinpackODS色谱柱 (4.6mm× 15 0mm) ,甲醇-水 (6 2∶38)为流动相 ,检测波长 2 5 4nm。结果 平均回收率为 98.0 4 % ,RSD =1.71%。结论 所建方法准确 ,可靠 ,可用于该药的质量控制。

OBJECTIVE To establish an HPLC method for determination of imperatorin in Tongqiao Biyan capsules.METHODS Shimpack ODS column (4.6 mm×150 mm)was used;The mobile phase was methanol-water(62∶38),and detecting wavelength was 254 nm.RESULTS The average recovery was 98.04%,RSD=1.71%.CONCLUSION The method was accurate and reliable. It could be used for determination of imperatorin in Tongqiao Biyan capsules.