聚合酶链反应在口岸鼠疫监测中的特异性和敏感性试验

The test of specificity and sensitivity about PCR technique in the port detection of Y.pestis

目的利用UDG防止聚合酶链反应(PCR)的产物污染,以2个不同基因为靶序列,设计了3对引物进行PCR扩增。方法用不同的模板考察方法的特异性和灵敏性。结果该方法可以特异性的检出鼠疫菌,对于DNA模板,检测灵敏度可以达到7CFU。结论PCR技术是一种快速、敏感、特异性很高的方法。

Objective A study on the specificity and sensitivity about PCR of Y.pestis. Method UDG was used for anti-carryover PCR product.Three primes were desigend for target seguence of two different gents. Result The sensitivity of the PCR which examined Y.pestis is 7CFU. Conclusion The PCR of the primers are of good specificity and sensitivity.