转Bt基因棉花主要抗虫黄酮类化合物时空动态的HPLC分析

HPLC analysis of main flavonoid chemicals and their spatio-temporal dynamics in Bt transgenic cotton.

棉花植株中的黄酮类化合物是重要的抗病虫害物质 .运用高效液相色谱技术 ,对转Bt基因棉花主要抗虫黄酮类化合物的种类、含量和时空动态进行了初步探讨 .结果表明 ,棉花组织中主要抗虫黄酮类化合物 (包括异槲皮苷、芸香苷和槲皮素等 )能够用HPLC方法检测并进行定量 ;异槲皮苷、芸香苷和槲皮素的含量均以花瓣中最高 ,花萼、苞叶和棉铃中较少 ;棉花生长中后期顶端嫩叶中抗虫黄酮类化合物的含量明显高于苗期 .不同组织不同生长期的主要抗虫黄酮类化合物含量有一定的差异 ,所起的抗虫作用也有所不同 .

Flavonoids are important chemicals of resistance to pests in cotton plant. The main flavonoid chemicals and their spatio temporal dynamics of content in Bt transgenic cotton were tested by HPLC. The results showed that the flavonoid chemicals of resistance to pests mainly including rutin, isoquercitrin and quercetin could be detected and quantitatively analyzed by HPLC. The contents of rutin, isoquercitrin and quercetin were the highest in petal, but lower in calyx, bract and cotton boll. Moreover, the total content of flavonoid chemicals in top leaf was much higher at developmental stage than at seedling stage. The content of each flavonoid chemicals of resistance to pests was different during different developmental stage and in different organs. It was indicated that different flavonoid chemicals played different roles in resistance to pests.