抗-耐甲氧西林金黄色葡萄球菌抗体的体外抑菌作用

Effect of Anti-MRSA Antibody on Meticillin-resistant Staphylococcus aureus in vitro

目的 研究抗 -耐甲氧西林金黄色葡萄球菌 (MRSA)抗体的体外抑菌作用。方法 通过对青紫蓝家兔进行接种 MRSA后产生抗 - MRSA抗体 ,采全血分离血清 ,用改良双向免疫扩散法进行抗体滴度测定 ;以正常血清为对照 ,在含 MRSA的培养基上作体外抑菌试验。结果 实验发现血清抗 - MRSA抗体效价为 1∶ 3 2 ,体外抑菌试验结果显示 :抗体组菌落数明显少于对照组 (P<0 .0 5 )。结论 抗 - MRSA抗体在体外对 MRSA有明显抑制作用 ,它将对

OBJECTIVE To study the antibacterial effect of anti MRSA antibody against meticillin resistant Staphylococcus aureus in vitro. METHODS The serum containing anti MRSA antibody was obtained from the rabbits after injected subcutaneously meticillin resistant S. aureus isolated from the infected wounds of patients. The titer of anti body was detected with modified immunodiffusion. Anti bacterial tests were done on the culture medium on which MRSA grew and anti bacterial effects were compared between the experimental group and the control one. RESULTS Antibody titer was 1∶32. The counting of colony on medium of experimentol group was less than that of control one. CONCLUSIONS The antibacterial effect of anti MRSA antibody against meticillin resistant S. aureus in vitro was significant. It was suggested that anti MRSA antibody play an important role in prevention and treatment of MRSA infection.