应用复合PCR方法鉴定口腔菌斑标本中的耐甲氧西林葡萄球菌属

Molecular Identification of Meticillin-resistant Staphylococcus spp in Dental Plaque

目的 建立临床上快速鉴定耐甲氧西林金黄色葡萄球菌 (MRSA )的方法 ,防止在患者之间特别是高风险患者中的流行传播。方法 建立了一种快速鉴定牙菌斑中 MRSA及耐甲氧西林血浆凝固酶阴性葡萄球菌 (MR-CONS)的方法 ,对含 6mg/ L甲氧西林的葡萄球菌 (Staphylococcus) No.110琼脂培养基生长菌落进行多引物PCR以检测 fem A和 mec A基因 ,力图同时确定目标样本的甲氧西林耐药性和葡萄球菌的表型。结果 在 2 0 0例样本中检测出 MRSA6例、MRCONS2 9例 ,结果与并行的常规生化细菌鉴定完全一致。结论 显示该方法是一种能特异、快速鉴定口腔菌斑 MRSA和

OBJECTIVE To detect meticillin resistant staphylococci colonized patients specifically and rapidly among persons at high risk for meticillin resistant Staphylococcus aureus (MRSA) carriage in order to prevent the spread of MRSA. METHODS A PCR method of multiplex amplification of femA and mecA determinants using growth on Staphylococcus No.110 agar containing 6 mg/L oxacillin was evaluated for detection of MRSA and meticillin resistant coagulase negative Staphylococcus (MRCONS) in 200 dental plaque samples. RESULTS Of the specimens tested, 6 MRSA and 29 MRCONS isolates detected by multiplex PCR method were confirmed by parallel conventional bacteriological tests with no false negative results. CONCLUSIONS These data indicate that the multiplex PCR method is a useful and rapid alternative to routine culture for detection of MRSA and MRCONS in surveillance specimens from dental plaque.