nef RNA在人类免疫缺陷病毒早期感染检测中的应用

The Application of nef RNA in Early Detection of HIV Infection

目的 为进行人类免疫缺陷病毒 (HIV)早期感染的检测 ,预防医院感染。方法 以 nef基因引物 nef6,nef7和 β-肌动蛋白 (actin)基因引物 BA1 ,BA4在同一反应体系中对标本进行逆转录聚合酶链反应 ,用 Kodak DigitalScience1D软件对条带密度进行分析 ,并对 PCR产物进行 Southern印迹杂交 ,以确定 PCR反应所得条带为 nef基因扩增产物。结果 所检测的 6例 HIV- 1抗体阴性 P2 4抗原阳性患者标本均扩增出 HIV- 1nef和 β-肌动蛋白的基因产物 ,通过 Kodak Digital Science1D软件分析得到各标本条带的密度比值 ,Southern印迹杂交证明 PCR反应条带确实为 HIV- 1nef基因扩增片段。结论 该方法简便、经济 ,可作为 HIV早期感染检测的一种辅助手段 ,防止医院内

OBJECTIVE To early detec nef RNA in HIV infection in order to prevent nosocomial infection.METHODS The primers nef 6 and nef 7 for nef gene and the primers BA 1, BA 4 for gene β actin were used in the same system to detect the specimen with reverse transcription polymerase chain reaction.RESULTS The amplified gene production of HIV 1 nef and β actin were showed in all six HIV 1 antibody negative P24 antigen positive specimen. The density ratio for six specimens were obtained by the analysis of Kodak Digital Science 1D software and nef PCR products were verified by Southern blot hybridization.CONCLUSIONS This method is simple and economic, and is one of the accessory methods in study of HIV early infection and prevention of nosocomial infection.