大肠癌术中附加区域性化疗的实验研究

EXPERIMENTAL STUDY OF REGIONAL CHEMOTHERAPY IN COLON OPERATION

目的 :探索大肠癌术中附加区域性化疗的可行性和临床实用意义。方法 :用家兔做实验动物 ,A组为试验组 ,B组为对照组 ,各组 10只 ,体重为 2～ 3kg ,以左半结肠和门静脉汇入的肝脏为靶区。A组从肠系膜下动脉注入 5 -氟尿嘧啶 (2 0mg/Kg体重 )后 ,分别于 2、5、10、15和 2 5min切取降结肠和肝脏组织各 0 .5～ 1.0g为样品 ,采用紫外分光光度法测定 5 -氟尿嘧啶的含量 ;B组从兔耳缘静脉注入 5 -氟尿嘧啶 (2 0mg/Kg体重 )后 ,切取样品部位和时间与A组相同。结果A组检测值在各时间段分别大于B组 :肝脏为 18.80 ,2 6 .39,2 1.4 9,19.38,16 .15倍 ;结肠为 12 .6 3,18.82 ,11.5 1,7.6 4 ,8.71倍 ;P <0 .0 0 1。结论 :以大肠癌部位为靶区 ,选择其供血的肠系膜下动脉给化疗药可显著提高左半结肠癌区及肝脏药物浓度和对杀灭癌细胞的强度。

Objective:To determine the feasibility of regional chemotherapy in colon cancer operation and the clinical meaning.Method:The experiment was carried out in hares, which were separated into two groups. Group A was experiment group and group B was contral group. Hares in group A were infused 5-Fu from mesentery artery and hares in group B from costa artery of ears. Samples were cut down from left colon and liver after 2,5,10,15 and 25 minutes.Result:Contents of 5-Fu in group A were higher than those in group B, which were 18.80, 26.39, 21.49, 19.38, 16.15 times in liver and 12.63, 18.82, 11.51, 7.64, 8.71 times in colon.Conclusion:Regarding cancer region of colon as a target, infusing chemotherapy drugs from mesentery artery may increase content of drug in colon and liver and kill cancer cells better.