胸腺内抗原注射抑制小鼠神经移植排斥反应

Immunological tolerance induction of nerve transplantation in adult mouseby intrathymic injection of allogenic antigen

目的 探讨胸腺内注射异基因抗原在同种异基因神经移植免疫耐受中的作用。方法 自供体鼠C5 7BL/ 6(H - 2 b)的脾细胞中提取MHC(H - 2 b)抗原 ,注入受体鼠Balb/c(H - 2 b)胸腺内 ,两周后移植供体坐骨神经。共设 4组 :胸腺内注射组 (I组 )、自体移植组 (Ⅱ组 )、异体移植组 (Ⅲ组 )和异体移植加用免疫抑制剂组 (Ⅳ组 )。移植后 3周做单向混合淋巴细胞培养 (MLC) ,诱导迟发型超敏反应 (DTH)。结果 I组小鼠淋巴细胞对供体淋巴细胞刺激的反应性减弱 ,DTH反应性降低。结论 胸腺内注射异基因H一

Objective To study the effects of intrathymic injection of allogenic antigen on immunological tolerance-inducing in nerve transplantation Methods MHC(H-2 b)antigen was extracted from donor C57BU6mouse splenic cells and injected intrathymieally into recipient Balb/c mouse(H-2 d),sciatic nerve Was transplanted after 2 weeks.Mice were divided into four groups,intrathymic injection group(Group Ⅱ),autogenictransplantation group(Group Ⅱ)and allogenie transplantation group(Group Ⅲ)and allogenie transplantationand immunosuppresion medicine group(Group Ⅳ).MLC and DTH were detected after another 3 weeks.Resuits MLC detection showed that the splenic cells of groupⅠmice to donor stimulator were significantly reduced and DTH WaS inhibited.Conclusion The intrathymie injection of donor H-2 antigen could induce immunologieal tolerance of nerve transplantation.