血糖安的细胞毒理学实验研究

Toxicity of XTA on guinea pig myocardial cells

目的 探讨血糖安 (XTA)对心肌细胞的毒性作用及其机制。方法 采用循环灌流的方法急性分离成年豚鼠心肌细胞 ,使其在血糖安浓度为 1μmo1 L、10 μmo1 L、10 0 μmo1 L、1mmo1 L作用后 ,分别检测 30、6 0、12 0、180min 4个时相心肌细胞保存液中LDH、CK含量 ,心肌细胞蛋白含量和Na+ K+ ATP酶活性 ;检测心肌细胞凋亡率和细胞活力 ;电镜下观察死亡和凋亡细胞的形态学变化。结果 在血糖安 10 0 μmo1 L和 1mmo1 L两个组的各时相保存液中 ,LDH、CK以及心肌细胞中蛋白含量明显增加 ;心肌细胞中Na+ K+ ATP酶活性和细胞活力明显降低 ,与对照组相比有显著性差异 (P <0 .0 1)。结论 在血糖安浓度高于 10 0 μmo1 L且作用时间在 30min以上时 。

Objective To study the toxicity of XTA on ventricular myocardial cells and its mechanism.Methods Cyclical perfusion was acutely used to detach the mature guinea pig myocytes.The activity of CK and LDH in a medium of myocardial cells was measured at 30,60,120 and 180 min after treated by XTA of 1μmol/L,10μmol/L, 100μmol/L and 1mmol/L.Rates of cell viability and apoptosis were also measured .The protein content and Na + K +ATP enzyme activity of the myocardial cells were measured as well.An electron microscope was used to investigate cell nucleus and organelle morphology.Results The release of CK,LDH and protein from the myocardial cell were increased gradually after administering XTA of 100μmol/L and 1mmol/L to the cells; cell activity decreased and the activity of Na + K + ATP enzyme decreased gradually. There were significant differences between XTA of 100μmol/L,1mmol/L and control group( P <0.01).Conclusion Gui nea pig ventricular myocardial cells are only injured after administering 100μmol/L and 1mmol/L concentrations of XTA higher than the effective concentration of the drug for 30min.