HBV前C区基因变异与血清肝纤维化标志物的相关性研究

Study on the relationship of HBV precore mutation with serum hepatic fibrosis markers

探讨HBV前c区基因变异与肝纤维化的相关性。应用聚合酶链反应-单链构象多肽性分析(PCR-SS-CP)银染技术检测FIBV前C区基因变异和放射免疫法检测血清肝纤维化标志物(血清透明质酸,HA和血清Ⅲ型前胶原肽,PCⅢ)检测103例HBeAg阴性而HBV-DNA阳性的慢性乙肝患者的血清,进行前C区基因变异与血清肝纤维化标志物水平的相关性分析。结果发现,70.87％发生了HBV前C区变异,其中包括变异株与野生株混合感染的病例,而这些病例中的血清肝纤维化标志物(HA、PCⅢ)水平显著高于单纯野生株感染者(P<0.01),HBV前C区基因变异检出率与血清肝纤维化标志物水平呈正相关,提示HBV前C区基因变异与肝纤维化密切相关。

Study on the relationship between HBV precore mutation and hepatic fibrosis in the serum of 103 patients with HBeAg ( - ) and HBV - DNA( + ). The precore mutation were analyzed by PCR- SSCP, and the serum levels of HA, PCⅢ were detected by radioimmunoassay. The results showed that 73 of them (70.87 % ) had the precore mutation, and its serum levels of HA, PCⅢwere higher in the patients with precore mutation than that in the patients without precore mutotion. The results suggest that the precore mutation might be related with hepatic fibrosis.