人游离毛囊体外培养及动态观察

In vitro culture of human hair follicles and sequential observation

目的进行人发毛囊的体外培养,并对其中后期改变进行形态学、组织学观察。方法将美容除皱术中切除的头皮在无菌条件下分离出游离的毛囊,培养于含血清DMEM培养基中,动态观测毛囊的生长速度及形态学、组织学改变。结果成功进行了人发毛囊的体外培养,毛囊培养中后期呈退化期改变。结论在含血清DMEM培养基中生长的毛囊易较早进入退化期,简化了游离毛囊的常规病理制片方法。

Objective To obtain successful in vitro culture of human hair follicles, and observe their morphological changes in the middle and later period of culture. Methods Human hair follicles were isolated from the scalp resected from patients receiving surgical treatment of the wrinkles, and were cultured in DMEM supplemented with 10% calf serum for up to 30 d. The growth of the follicles, along with their morphological and histological changes, was observed sequentially. Results In vitro culture of human hair follicles was successfully obtained. The follicles showed catagen-like changes in the middle and later period of culture. Conclusion Human hair follicles cultured in vitro tend to transform early into catagen, and this culture method has simplified preparation of routine paraffin section of hair follicles.