动态张应变下大鼠肌卫星细胞三维培养的形态学观察

Morphological study on three-dimensional culture of rat muscle satellite cells under cyclic mechanical stretch

目的探讨三维培养条件下动态张应变对大鼠骨骼肌卫星细胞形态学的影响。方法将牵张应变(10%应变、频率0.2 Hz,每小时加载10 min,共作用48 h)作用于大鼠骨骼肌卫星细胞-支架材料复合物。结果在动态张应变作用下,骨骼肌卫星细胞沿应力方向延伸,并见到肌小管形成;而未施加应力的骨骼肌卫星细胞在各个方向上都有突起,但未见到肌小管形成。结论动态张应变有利于肌纤维理想极性的形成,并有助于组织工程化骨骼肌的构建。

Objective To study the effect of mechanical stretch on the morphologic change of rat muscle satellite cell in the three-dimensional culture. Methods Based on the apparatus for three-dimensional cell cultures under a cyclic mechanical strain, a specific stretch pattern (10% elongation, 10 stretches/min for 10 min of each hour,48 h total) was applied to cell-scaffolding composites. Results Under the stretch pattern, the shape of satellite cells changed to oblate and spread to the direction of the stretch. Furthermore, the myotube was observed. On the contrary, the satellite cells spread to the all direction, and the formation of the myotube was not been found in the control group. Conclusion Cyclic mechanical stretch is helpful for the formation of the ideal directivity and these results are compatible with a significant role for the stretch in tissue-engineered muscle construction.