三种不同免疫抑制剂对胰岛细胞功能的影响

Effects of three different immunosuppressive drugs on SD rat islet cell viability

目的观察免疫抑制剂对体外培养胰岛细胞活力的影响,探讨免疫抑制剂对胰岛移植物功能的影响。方法应用体外纯化后培养的SD大鼠胰岛细胞,采用噻唑蓝法测定在不同浓度免疫抑制剂作用下胰岛细胞活力的变化。结果低剂量雷帕霉素对胰岛细胞活力无明显影响,1 ng/ml以上雷帕霉素能引起胰岛细胞活力降低;低剂量和高剂量daclizumab和FTY720对胰岛细胞的活力均无明显影响。结论雷帕霉素对胰岛细胞有直接损伤作用,这种损伤程度与浓度呈正相关,daclizumab和FTY720对胰岛细胞无明显毒性。

Objective To investigate the effect of immunosuppressive drugs on the viability of pancreatic islet cells cultured in vitro, and to explore their effects on the outcome of islet transplantation. Methods Pancreatic islet cells from SD rats were cultured in vitro, and the changes in their viability determined by MTT assay after incubation with immunosuppressive drugs at different concentrations. Results The viability of the islet cells was reduced after exposure to rapamycin at higher concentrations (≥1 ng/ml), but not at lower concentrations. Treatment with daclizumab and FTY720, at either lower or higher concentrations, did not result in significant reduction of the islet viability. Conclusions Rapamycin may directly cause damage to islet cells, the degree of which is positively correlated to the concentration of rapamycin. No obvious toxicity of daclizumab and FTY720 was observed against the islet cells.