酪氨酸蛋白激酶受体B在人卵泡中的表达及意义

Expression of tyrosine kinase receptor B in human follicle

目的探讨酪氨酸蛋白激酶受体B(tyrosine kinase receptor B, TrkB)在人卵泡中的表达及意义。方法用免疫细胞化学方法检测TrkB在超促排卵妇女壁层颗粒细胞、卵丘颗粒细胞、未受精或未成熟卵母细胞中的表达,并用Western免疫印迹方法检测TrkB在壁层颗粒细胞、卵丘颗粒细胞中的表达水平。结果壁层颗粒细胞、卵丘颗粒细胞、卵母细胞均有TrkB表达。壁层颗粒细胞表达量高于卵丘颗粒细胞。结论脑源性神经营养因子在人卵巢可能通过与TrkB结合,参与颗粒细胞功能的完成和卵母细胞发育的调控。

Objective To study the expression of tyrosine kinase receptor B(TrkB) in human follicle and explore the significance of the expression. Methods Streptavidin-biotin peroxidase complex immunocytochemistry was employed to determine the expression of TrkB on the oocytes, cumulus granulosa cells (CGCs) or mural granulosa cells (MGCs) obtained from women during controlled ovarian hyperstimulation for in vitro fertilization-embryo transfer (IVF-ET). Western blotting was used to analyze TrkB expression level in CGCs and MGCs. Results The results demonstrated for the first time the presence of TrkB on the surface of human ovary cells including MGCs, CGCs and the oocytes. Higher expression levels of TrkB were detected on the MGCs than on the CGCs. Conclusion It is possible that TrkB tyrosine autophosphorylation in response to neurotrophins can take part in the regulation and modulation of steroidogenesis and oocyte maturation.