血浆vW因子抗原检测在儿童感染性休克中的意义

The clinical significance of von Willebrand Factor in children with septic shock

目的 探讨血浆vonWillebrandFactorAntigen(vWf :Ag)在儿童感染性休克中的临床意义。 方法 用ELISA方法检测 9例休克死亡组、11例休克存活组及 10例健康对照组患儿血浆vWf:Ag(vW因子抗原 ) ,同时用酶比色法检测这 2 0例感染性休克患儿的动脉血乳酸。 结果 血浆平均vWf :Ag休克死亡组 (710 9± 3 9 4) %明显高于休克存活组 (5 94 5± 5 8 6) % (P<0 0 0 0 1)和健康对照组 (93 8± 16 1) % (P <0 0 0 0 1)。 2 0例休克患儿血浆平均vWf:Ag(64 6 9± 77 3 9) %与平均动脉血乳酸浓度 (3 96± 1 0 0 5 )mmol/L呈正相关 (r =0 886) ,与器官衰竭数目亦成正相关 (rs=0 92 9)。 结论

Objective To investigate the clinical significance of von Willebrand Factor Antigen (vWf:Ag) in children with septic shock.Methods Thirty children (20 with septic shock and 10 healthy controls) were enrolled in the study.Blood samples were obtained for plasma vWf:Ag and arterial lactate on Day 0 as soon as possible after diagnosis.The plasma vWf:Ag and arterial Lactate concentration were determined by an enzyme linked immunosorbant assay and enzyme colorimetric analysis respectively.Results Septic shock non survivors had significantly greater mean vWf:Ag (710 9±39 4)% than septic shock survivors (594 5±58 6)%( P <0 0001) and controls (93 8±16 1)%( P <0 0001). The mean vWf:Ag(646 9±77 39)% of 20 patients with septic shock were positive correlation to mean arterial lactate concentrations (3 96±1 005)mmol/L( r=0 886) and the number of organ failures (r\-s=0 929).Conclusion The measurement of plasma vWf:Ag in septic shock may be a useful indicator of the severity of endothelial damage and the development of multiple organ system failure.