烧伤大鼠肺泡巨噬细胞CD14蛋白表达对细胞因子的影响

Effects of mCD14 protein expression on cytokines in early burned rat

目的 :探讨严重烧伤对大鼠肺泡巨噬细胞 (AM)CD14膜蛋白 (mCD14)表达变化及其对AM分泌细胞因子的影响。方法 :2 0 %Ⅲ度烧伤大鼠检测早期外周血LPS浓度。AM以烧伤血清及LPS刺激 ,再分别以抗CD14抗体作用 ,免疫组化检测mCD14蛋白表达变化 ;ELISA检测培养液中TNFα和IL - 6浓度。结果 :烧伤血清和LPS刺激后 ,mCD14蛋白表达从 1h起就开始增加 ,2h达峰值 ,尔后逐渐减弱 ,上述改变在 12h内持续 ,细胞因子分泌相应增加 ;抗CD14抗体阻断CD14作用后 ,mCD14的蛋白表达显著降低 ,细胞因子分泌亦相应减少。结论 :严重烧伤后随LPS增加 ,激活内毒素信号传导通路 ,使AM分泌细胞因子增加 ,此作用可以被抗CD14抗体所阻断 。

Objective:To observe AM mCD14 protein expression and the cytokine(TNFα,IL-6) concentration of severely burned rat in early stage.Methods:SD rat were burned 20%TBSA Ⅲ oinjury and the early stage LPS concentration in blood were assayed . AM were isolated at 1,2,3,4,6,8,12 h after burned. mCD14 protein expression were assayed by immunohistochemical method after burn. TNF-α and IL-6 concentration of the culture liquid were assayed at the same time.Results: mCD14 expression was found increasing significantly in alveolus macrophages. These effects peaked at 2 h and lasted for 12 h.At the same time, TNF-α,IL-6 concentration of culture liquid elevated correspondingly. when CD14 antibody was used, the expression of mCD14 decreased significantly.TNF-α,IL-6 concentration decreased at the same time.Conclusion:AM mCD14 expression upregulated after burn. This change may related with the increased concentration of LPS and the activated LPS pathway. Such regulation may be blocked by the CD14 antibody and decrease the TNF-αand IL-6 concentration.