快速建立继发性脾功能亢进的犬动物模型

Rapid establishment of a canine model of secondary hypersplenism

目的 介绍一种快速建立的继发性脾功能亢进的犬动物模型。方法 通过结扎犬的脾静脉主干和脾静脉属支引起淤血性脾肿大 ,观察动物外周血细胞变化 ,了解脾脏影像学变化以及组织病理学改变。结果 脾静脉结扎后 1周外周血红细胞、血小板即下降 ,第 3周以后二者下降明显 (P <0 0 5 ) ,红细胞和血小板减少、脾肿大可持续 9周以上 ;术后第 2周起 ,脾脏组织学改变符合脾淤血改变 ;脾切除术后红细胞和血小板逐渐恢复正常。脾静脉结扎后白细胞水平无显著改变。结论 脾静脉结扎建立继发性功能亢进方法简单、确切 ,可以作为脾功能亢进外科或介入治疗的较理想模型。

Objective To introduce a method for the rapid establishment of a canine model of secondary hypersplenism. Methods Congestive splenomegaly was induced by ligation of splenic vein and its collateral branches in mongrel dogs. The blood cell counts for peripheral venous blood were determined weekly, the data of spleen size examined by radiological examinations and histopathological changes of spleen observed regularly. Results The erythrocyte and platelet counts decreased in the first postoperative week after splenic vein ligation, and were significantly lower at the end of 3rd week ( P <0.05) and thereafter sustained. The splenomegaly, erythrocytopenia and thrombocytopenia remained over 9 weeks. Since the 2nd week after splenic vein ligation, the splenic histopathological changes conformed to the changes of congestive splenomagely. The low levels of erythrocyte and platelet counts were recovered gradually and approached similarly to the levels in the control group at two weeks after splenectomy. No significant changes of the leukocyte counts were observed after splenic vein ligation throughout the experiment. Conclusion The method of splenic vein ligation to induce experimental secondary hypersplenism is a simple, effective and relatively ideal model for surgical or interventional therapy in hypersplenism.