TNFα对烫伤大鼠骨骼肌26S蛋白酶复合体及19S调节复合体的作用

Effects of TNFα on 26S proteasome and 19S regulator in skeletal muscle of the severely scalded rats

目的 为阐明及防治烧伤后负氮平衡提供理论依据。方法 在接近体内环境下于体外分别用免疫沉淀扣除法和间接ELISA法检测TNFα对 3 0 %体表皮肤全层烫伤大鼠烧伤后第 1、2、3、5、7天内骨骼肌中 2 6S蛋白酶复合体及 19S调节复合体的活性和蛋白水平的影响。结果 TNFα可以刺激烧伤后大鼠骨骼肌中 2 6S蛋白酶复合体及 19S调节复合体的活性和蛋白表达水平明显升高。结论 TNFα对烧伤后大鼠骨骼肌中 2 6S蛋白酶复合体系统的激活具有重要作用 ,从而加强蛋白质的分解代谢 ,这可能与烧伤后负氮平衡的发生密切相关。

Objective To explore the mechanism of negative nitrogen balance in the treatment after burns. Methods The means of immuno precipitation deduction and ELISA were used to test the activities and contents of 26S proteasome and 19S regulator in skeletal muscle of rats inflicted with 30%TBSAⅢ burns. Results TNFα markedly raised the activities and contents of 26S proteasome and 19S regulator in skeletal muscle after scalding. Conclusion TNFα activates the 26S proteasome system in skeletal muscle, thus it enhances the degradation of protein, which is associated with the development of negative nitrogen balance following scalding.