TGF-β_1在肺鳞癌细胞中的表达

Expression of TGF-β_1 in human pulmonary squamous cell carcinoma

目的 探讨TGF β1 在肺鳞癌细胞中的表达。方法 以原代培养的人肺鳞癌细胞为对象 ,采用ELISA法检测不同分化程度肺鳞癌细胞无血清培养液中TGF β1 蛋白含量。结果 肺鳞癌各组细胞中TGF β1 蛋白含量均显著高于正常支气管上皮细胞 (P <0 0 1) ,低分化组分别高于中分化组和高分化组 (P <0 0 5 ) ,中分化组虽高于高分化组 ,但无显著差异(P >0 0 5 )。结论 TGF β1 蛋白在人肺鳞癌组织中表达增高 ,且与其分化程度基本呈正相关 ,提示TGF β1

Objective To investigate the expression of TGF β 1 in human pulmonary squamous cell carcinoma Methods TGF β 1 in serum free conditioned medium of different differentiated degree primary cells in culture from human pulmonary squamous cell carcinoma were detected by ELISA Results TGF β 1 in serum free conditioned medium from all tumor cell groups is significantly higher than from normal bronchial epithelial cell groups ( P <0 01), and poorly differentiated tumor cell group is significantly higher than well or moderately differentiated tumor cell groups respectively ( P <0 05), but the difference between well and moderately differentiated tumor cell groups is not significant ( P >0 05) Conclusion Inhibition effect by TGF β 1 on cell growth of human pulmonary squamous cell carcinoma is weakened