血管紧张素转换酶基因多态性和血管紧张素Ⅱ受体-1基因多态性与原发性高血压

Angiotensin Converting Enzyme Gene and AngiotensinⅡTypeⅠReceptor Gene Polymorphism in Essential Hypertension

目的 探讨血管紧张素转换酶 (angiotensinconvertingenzyme ,ACE)基因多态性和血管紧张素Ⅱ受体 - 1(AT1R)基因多态性与原发性高血压 (EssentialHypertension ,EH)的关系 ,以及这两种基因多态性对于EH的发病是否有协同作用。方法 对 74 0名开滦集团公司职工进行 10年的纵向研究 ,应用聚合酶链反应和限制性酶切方法检测ACE基因第 16位内含子插入 /缺失 (I/D)多态性分布及AT1R基因A116 6C多态性分布。结果 10年前或 10年后EH组的ACE各基因型及等位基因频率与同期正常血压组比较无显著差异。 10年前或 10年后EH组的AT1R各基因型及等位基因频率与同期正常血压组比较无显著差异。 10年前或 10年后EH组中同时具有DD基因型和AC基因型的频率分布与同期正常血压组比较均无显著差异。 10年前或 10年后的男性EH亚组中 ,同时具有DD基因型和AC基因型的频率分布与同期正常血压组比较有统计学差异。结论 血管紧张素转换酶基因的I/D多态性和血管紧张素Ⅱ受体 - 1基因A116 6C多态性与原发性高血压无关 。

Objective To investigate angiotensin converting enzyme (ACE) gene and angiotensin Ⅱ type Ⅰ receptor (AT 1R) gene polymorphism in essential hypertension. Methods 740 subjects from the employees of Kailuan Coal Mines were randomly enrolled and were longitudinally studied for 10 years. I/D polymorphism of ACE gene and A1166C polymorphism of AT 1R gene was examined by polymerase chain reaction (PCR) and PCR-based restriction enzyme analysis method, respectively. Results The distribution of ACE and AT 1R genotypes and alleles showed no difference between hypertensives and controls before and after 10 years. However, the frequencies of combined genotypes of AC and DD were significant difference in males between hypertensives and controls. Conclusion There was no significant difference in the distribution of ACE and AT 1R genotypes between hypertensives and controls before and after 10 years. A synergistic effect between the genotypes of AC and DD was apparent in males with hypertensives.