高强度聚焦超声破坏膀胱癌细胞株的实验研究

The study of bladder cancer cell line irradiated with high intensity focused ultrasound

目的:探讨高强度聚焦超声(HIFU)治疗膀胱癌的可行性。方法:HIFU辐照T_(24)膀胱癌细胞株后用MTT法测定活细胞数,分析HIFU剂量与细胞存活率的关系,测定培养液内碱性磷酸酶(ALP)、肌酸激酶(CK)活性,并对癌细胞进行形态学观察。结果:随着HIFU剂量增加,膀胱癌细胞存活率迅速减少,残留癌细胞生长受抑。癌细胞结构受到不可逆性破坏。结论:HIFU治疗膀胱癌是可行的。

Objective:To investigate the feasibility and mechanism of high intensity focused ultrasound (HIFU) in the treatment of bladder cancer. Methods:After irradiating the bladder cancer cell line T24,the number of survival cells was detected with MTT col-orimetry. The relationship between HIFU dosage and cell survival rate was analysed. The activity of alkaline phosphatase (ALP) and creatine- kinase (CK) in culture medium was determined and the structures of the cells were studied. Results: The relationship between sound intensity and cell survival rate is in power series. With increasing sound intensity ,cell survival rate decreased sharply. The relationship between irradiation time and cell survival rate is linear. When sound intensity was 2300 W/cm2, the optimum irradiation time was 7 seconds. After irradiation ,cell growth was suppressed and cell numbers reduced continuously until 6 hours later. This indicated that there was necrobiosis in these cells. With the prolongation of the irradiation, the activity of ALP、CK in culture medium increased and reached it's peak at two seconds. Under optical and electronic microscopes, the damage to the cancer cell was irreversible. Conclusion :HIFU is an effective ,minimally invasive treatment option for bladder tumor.