卡介菌多糖核酸对肺癌细胞与脐静脉血管内皮细胞粘附力学特性影响

The effect of BCG - PSN on the mechanical properties of adhesion between PG cells or PAa cells and HUVEC

目的:本文从生物力学角度揭示在卡介菌多糖核酸(BCG-PSN)作用下,肺癌细胞与脐静脉血管内皮细胞(HUVEC)的粘附力学特性。方法:采用微管吸吮技术定量测定低转移人肺腺癌(PAa)细胞和高转移人肺巨细胞癌(PG)细胞与脐静脉血管内皮细胞的粘附力(Fa)及相对粘附应力(S_1);并观察在卡介菌多糖核酸(BCG-PSN)作用下,Fa和S_1变化。结果:①肺癌细胞与HUVEC的粘附力(Fa)PG细胞大于PAa细胞,用BCG-PSN后,其在PAa细胞呈浓度依赖性及时间依赖性下降,在PG表现在50μg/ml及以上浓度组粘附力呈明显浓度依赖性下降。②肺癌细胞与脐静脉血管内皮细胞的S_1与Fa的变化有较好的一致性。结论:实验条件下PG细胞与HUVEC的粘附力大于PAa的细胞,且两种细胞与内皮细胞的粘附力均受BCG-PSN的抑制,表明PG细胞具有较高的血行转移潜能及BCG-PSN抗肿瘤作用。

Objectives :To investigate the effect of BCG - PSN on the mechanical properties of adhesion between lung cancer cells and human umbilical vein endothelial cells. Methods: Micropipette aspiration technique was used to investigate quantitatively the adhesion force (Fa) and the relative adhesion stress (S1) between high metastatic human lung giant cell carcinoma (PG) cells or low metastatic lung adenocarcinoma (PAa) cells and human umbilical vein endothelial cells (HUVEC). Further,we Invistgated the effect of bacillus of Calmette and Guerin - polysaccharide nucleic acid (BCG - PSN) on Fa and S1. Results: The adhesion force (Fa) of PG cells to HUVEC were higher than that of PAa cells under the experimental condition. With treatment using BCG - PSN, the values of the adhesion force of PAa cells to HUVEC decreased in a concentration - dependent and time - dependent manner. For PG cells, treatment with a concentration of 50μg/ml or higher resulted in a continuous decrease of the cell adhesion force. And the adhesion force to HUVEC decreased continuously with the duration of treament from 30 minutes to 48 hours, but they increased significantly again in 72 hours after treatment. The relative adhesion stress between lung cancer cells and HUVEC,S1,changed almost in the same tendency as the adhesion force,Fa. Conclusion :The adhesion forces of PG cells to HUVEC were higher than that of PAa cells under the experimental condition. The adhesion forces of both of the lung cancer cells to HUVEC can be inhibited by BCG - PSN. These results might be relative to the high metastatic potential of PG cells through blood circulation, and to the antitumor action of HCG-PSN.