茶多酚、维生素C对镉急性毒性影响的实验研究

Experimental study on the effect of tea polyphenols and Vitamin C on acute toxicity of cadmium

目的 研究预先投予茶多酚 (TP)和VitC对急性镉 (Cd)毒性的影响。方法 4组Wistar大鼠分别皮下注射氯化钠、 2 5 μmol/kg的氯化镉溶液 ,以及以TP、VitC预处理后的 2 5 μmol/kg氯化镉溶液。染毒 2 4h后测定其血清中的ALT和LDH含量 ,肝和肾皮质的Cd含量 ,肝、肾组织中的GSH和MDA含量及GSH Px活性。结果 与对照组比较 ,单纯染镉组大鼠肝、肾Cd含量升高 ,肝GSH、MDA含量升高 ,GSH Px活性降低 ,而肾GSH Px活性降低。经TP和VitC预处理后 ,急性染镉大鼠血清中的ALT和LDH活性及肝脏中GSH、MDA含量明显低于单纯染镉组 ,而GSH Px活性高于单纯染镉组。TP干预组肝脏中的Cd及肾脏中MDA显著低于单纯染镉组 ,VitC干预组未见差异。与单纯染镉组比较 ,TP和VitC干预组肾脏中的Cd、GSH含量及GSH Px活性差异无显著性。

Objective To study the effects of tea polyphenols(TP)and Vitamin C(Vit C) on acute toxicity of cadmium(Cd).Method 40 Wistar rats were randomly divided into four groups.The rats of control were injected subcutaneously with normal saline.The rats in second group were subcutaneously injected with CdCl 2 25 μmol/kg.The rats in third group and fourth group were pretreated with TP and Vit C,respectively and then injected subcutaneously with 25 μmol/kg CdCl 2,activities of ALT and LDH in serum,levels of cadmium,GSH,MDA and GSH-Px activity in the liver and renal cortex of the rats were determined 24 hours after administration of cadmium.Result Compared with controls,serum ALT and LDH activities,cadmium levels in liver and renal cortex,GSH,MDA concentration in liver were all increased,GSH level decreased in renal cortex;GSH-Px activities in liver and renal cortex were decreased with the cadmium dose of exposure.While serum ALT and LDH activities and the hepatic GSH and MDA level in the rats pretreated with TP and Vit C were significantly lower than those of injecting cadmium only,but the GSH-Px activities in liver were higher than Cd exposed rats;the cadmium level in liver and MDA concentration in renal cortex of rats pretreated with TP were significantly lower than cadmium treated rats;the differences of Cd,GSH,GSH-Px in renal cortex between TP and Vit C pretreated group and cadmium group had no significance.Conclusion Acute exposure to cadmium may mainly cause liver damage,pretreatment with TP and Vit C can protect the liver from damage caused by cadmium.