结直肠癌腹腔转移与CK20 mRNA、CD44v6和P53表达及意义

The association of the expressions of CK20 mRNA, CD44v6 and P53 with abdominal cavity metastasis of colorectal cancer and the related significance

目的 研究结直肠癌腹腔转移与CK2 0mRNA、CD44v6和P5 3的表达及意义。方法 采用逆转录 聚合酶链反应 (RT PCR)技术 ,对 5 0例结直肠癌进行腹腔渗液CK2 0mRNA测定 ,并且对癌组织行免疫组化检测CD44v6和P5 3。结果 CK2 0mRNA阳性表达与癌组织中P5 3和CD44v6之间存在显著性差异。结论 检测腹腔渗液中的CK2 0mRNA并结合癌组织中P5 3和CD44v6的检测 ,对于预测结直肠癌腹腔转移和指导术后治疗具有重要参考价值。

Objective To study the association of abdominal cavity metastasis of colorectal cancer with the expressions of CK20 mRNA, CD44v6 and P53 and the related significance. Methods CK20 mRNA in peritoneal fluid was detected by reverse transcription polymerase chain reaction (RT PCR) and proteins of CD44v6 and P53 in cancer tissues were examined immunohistochemically in 50 cases of colorectal cancer. Results Significant correlation was found between CK20 mRNA and the expressions of CD44v6 and P53 in cancer tissues. Conclusion The detection of CK20 mRNA in peritoneal fluid plus examination of CD44v6 and P53 in cancer tissues are helpful for the prediction of abdominal cavity metastasis of colorectal cancer and post operation treatment.