不同浓度的海藻酸盐与异种骨构建组织工程载体研究

CONSTRUCTION OF CARRIER FOR BONE TISSUE ENGINEERING WITH DIFFERENT CONCENTRATIONS OF ALGINATE CONBINED WITH XENOGRAFT

目的 探讨不同浓度的海藻酸盐凝胶与去抗原异种骨构建组织工程载体的生物学性能。方法 采用倒置显微镜 ,扫描电镜 ,对 0 .2 5、1、4和 8%的海藻酸盐与去抗原异种骨构建的组织工程载体中的骨髓基质细胞形态及分泌性能。结果 海藻酸盐的浓度为 0 .2 5 %和 1%时 ,凝胶在异种骨中均匀复合 ,浓度为 4 %和 8%时海藻酸盐仅复合在松质骨表面。体外培养 4天后 ,0 .2 5 %海藻酸盐复合异种骨的表面大部分凝胶脱落 ;1%海藻酸盐均匀复合在异种骨的骨孔中 ,细胞在凝胶中生长均匀 ,形态饱满 ,有基质分泌 ;4 %海藻酸盐中细胞生长受到限制 ;8%海藻酸盐凝胶质地不均匀 ,无法见到细胞结构。结论 1%海藻酸盐适宜与异种骨复合构建骨组织工程载体。

Objective To investigate bio characteristics of bone stromal cells (MSC) in different concentrations of alginate combined with xenograft. Methods The configuration and secretion of MSC in different concentrations of alginate combined with xenograft were observed by scanning electron microscope and inverted microscope. Results When the concentration of alginate was 0.25% or 1%, alginate was equally combined in xenograft, 4% and 8% only on the surface of xenograft. After cultured for 4 days, alginate of 0.25% came off from xenograft. But alginate of 1% was equally combined in xenograft with cell secreting well in alginate. The growth of cells in alginate of 4% was restricted and no cell was seen in alginate of 8%. Conclusion Alginate of 1% is suitable for constructing carrier of tissue engineering bone.