苯甲醛高耐受性酵母菌的选育

Screening of Saccharomyces cerevisiae strains with high tolerance to benzaldehyde for L-PAC production

介绍一种经过长期诱导、驯化作用来选育耐性菌株的方法。通过固定化细胞的间歇补料培养方式和长期诱导、驯化后 ,筛选出 8株具有较高苯甲醛耐受性的酿酒酵母菌株 ,其中菌株Sbht_35_2 3对苯甲醛的耐受性达到 0 .9% ,并保持较高的稳定活性。采用这些具有较高耐性的酿酒酵母菌株生物合成L_苯基乙酰基甲醇 (L_PAC) ,将有利于提高转化率。

Using immobilized Saccharomyces cereviesiae cells repeatedly inducted by benzaldehyde with reactivation by fed-cultivation,eight strains with high tolerance to benzaldehyde were obtained.The result showed that strain Sbht-35-23 was high tolerance to benzaldehyde and a high stability among all obtained strains.Application of such strains would benefit to the biotransformation of L-phenylacetylcarbinol by benzaldehyde and pyruvate.