通痹灵和其它六个方药对强直性脊柱炎成纤维细胞的作用对比

The Act Contrast Between Tongbiling and 6 Formulas to Fibroblasts of Ankylosing Spondylitis (AS) in Vitro

目的 :比较 7个治疗强直性脊柱炎 (AS)的临床方药对AS成纤维细胞的抑制作用 ,通过量化比较 ,以期筛选出最佳的治则方药 ,为临床中医药治疗AS提供指导或参考。方法 :采用AS髋关节囊体外培养AS成纤维细胞系 ,经传代培养 ,运用中药血清药理学方法 ,制备 7种AS有效代表方含药大鼠血清及对照组血清 ,分别通过四甲基偶氮唑盐 (MTT)法、羟脯氨酸 (HYP)比色法、12 5Ⅰ标记放射免疫 (RIA)法测定各含药血清对AS成纤维细胞的增殖、合成分泌胶原和骨钙素的影响 ,量化比较它们的抑制率。结果 :各组含药血清对AS成纤维细胞都有一定的药理作用。对细胞增殖抑制的比较 ,各组作用强弱依次为C >B >D >F >A >G >E ;对细胞外胶原合成抑制而言 ,作用强弱依次为B >D >C >F >A >G >E ;对骨钙素的排泌的抑制作用 ,则强弱依次为B >D >F >A >C >G >E。结论 :AS各有效代表方对AS成纤维细胞均有不同程度的抑制作用 ,综合分析 ,通痹灵 (治则 :扶正固本 ,化痰软坚散结 )、复方雷公藤煎剂 (治则 :祛风湿止痹痛 ,益肝肾补气血 )相对作用更强 ,因此 ,当临床运用中医药治疗AS ,以延缓或阻断病情发展时 ,更有借鉴参考价值。

Objective:Comparing inhibit ional effects to AS fibroblasts and to select the best principle of treatment to AS and representative formulas.Methods:Fibrous tissues were collected from AS patient's hips.The cells were cultured in vitro and normal tissue was collected from patients with traumatic bone necrosis as a control group.After subcultrue,fibroblasts were taken as reactive model.Using serum pharmacological method that the sera derived from the rats orally taking seven kinds of AS representative formulas (their code named A?B?C?D?E?F?G),were incubated with AS fibroblasts in vitro,respectively.To observe the effects of the serum on proliferation Capacity and synthesis of collagen and osteocalcin of two cell lines,MTT assay,HYP determination and 125 I radio immunoassay were carried out.Results:Each serum with herbs has effect to AS fibroblasts.On inhibit the cells proliferation,the seropharmacological effects of AS representative CMFs was C>B>D>F>A>G>E;while on the effects of reduce collagen synthesis,B>D>C>F>A>G>E;for reduction OCN synthesis,B>D>F>C>A>G>E.Conclusion:Different herbs formulas have inhabit ional effects to varying degrees,but Tongbiling (principle of treatment is on supporting the healthy,eliminating phlegm and dissipating a mass) and Complex prescription of regongten (dispelling wind-evil and wetness-evil,invigorating liver and kidney) have stronger pharmacological effects. KEY WORDS Ankylosing spondylitis Fibroblast Herbscrepancy either between squamous cancer cell and adenocarcinoma cell or between squamous cancer cell and small cell undiffereniated cancer cells. This difference also existed in adenocarcinoma cell and small cell undiffereniated cancer cells (P<0.05). Particles were bigger and stronger in adenocarcinoma than those in squamous cancer cell and small cell undiffereniated cancer cells. There was obvious relation between types of AgNOR particles and those of cancer cell (P<0.01). AgNOR sensitivity and specificity were 94.1% and 92.3% respectively by AgNOR count criterion. Conclusions AgNOR technique can help make diagnosis for which it is difficult to do so by routine staining. The types of cancer cell and AgNOR are correlative.