前列腺癌错配修复蛋白PMS1的实验研究

The study of protein PMS1 of DNA mismatch repair in human prostate cancer

目的 探讨错配修复基因(MMR)蛋白PMS1在前列腺癌细胞株及前列腺癌标本中的表达及其意义。方法 利用Western印迹法检测前列腺癌细胞株中错配修复蛋白PMS1的丢失情况;用免疫组织化学法检测前列腺癌标本中PMS1的表达。结果 在所研究的8个前列腺癌细胞株中均发现有错配修复蛋白PMS1的缺失;前列腺癌标本中也存在错配修复蛋白PMS1表达的减少或消失。结论 前列腺癌中错配修复蛋白PMS1的缺失是很普遍的,其缺失直接参与了前列腺癌的发生发展。

Objective To explore the expression and role of mismatch repair (MMR) protein PMS1 in prostate cell-lines and human prostate cancer specimens.Methods Using Western blot analysis, we detected the loss of PMS1 in prostate cell-lines. We also immunohistochemically analysis the expression of PMS1 in human prostate cancer specimens.Results We found the deletion of MMR in all prostate cell-lines and the reduction or absence of PMSl's expressions in human prostate cancer specimens. Conclusion From these results, we conclude that there are deletion of PMS1 in prostate cancer, which influence the origination and development of prostate can-