外源多胺对宁夏枸杞离体形态发生过程中可溶性蛋白组分和过氧化物酶同工酶酶谱的影响

Effects of polyamines and its synthetic inhibitors on soluble protein components and isoperoxidase patterns during in vitro morphogenesis of Lycium barbarum

利用已建立的宁夏枸杞 ( Lycium barbarum L.)器官发生和体细胞胚发生体系 ,对多胺在高等植物离体形态发生中的作用以及部分生化特征进行了研究 .将不同多胺预处理过的愈伤组织诱导器官发生的实验中 ,Spd能显著提高不定芽的再生频率 ,而 CHA的预处理对不定芽形成产生的阻碍最为严重 .在体细胞胚发生体系中 ,Spd,Put的处理能促进体细胞胚成苗 ,而 MGBG,CHA的处理明显降低了成苗率 .不同的多胺及其生物合成抑制剂均能影响两种再生体系的可溶性蛋白组分和过氧化物酶 ( POD)同工酶谱的表达 ;但对器官发生和体细胞胚发生途径特异多肽 ( 4 6.3,36.2 k D)的合成没有明显影响 ;而对存在于器官发生全过程、仅出现于体细胞胚发生后期阶段的 4 4k D多肽 ,MGBG在两种途径中都能阻碍它的表达 ,Put仅在体细胞胚发生中有抑制作用 .这些结果显示多胺可能在高等植物离体形态发生中起“第二信使”作用 .

By using the established system of organogenesis and somatic embryogenesis of Lycium barbarum L., effects of polyamines (PAs) and its biosynthetic inhibitors on in vitro morphogenesis and changes of some biochemical characters were studied. When calli pretreated with different PAs were induced for organogenesis, Spd markedly increased regenerative rate of adventitious bud and treatment of CHA had the most striking effect of inhibition on organogenesis. During somatic embryogenesis of pretreated calli, plant regeneration was enhanced by Spd and Put and inhibited by CHA and MGBG. All of PAs and its biosynthesis inhibitors affected the expression of soluble protein components and isoperoxidase patterns in both regenerative systems, but the syntheses of specific peptides (46.3,36.2 kD) during organogenesis and somatic embryogenesis were not changed apparently. To 44 kD peptide that expressed throughout progress of organogenesis and appeared only in the late stage of somatic embryogenesis, MGBG could block its expression in two development pathways, while Put had an inhibitory effect on its expression only in somatic embryogenesis. The results showed that PAs could play a role as a 'Second messenger' during in vitro morphogenesis in higher plants.