小鼠胚泡着床相关基因的差减筛选

Screening of Mouse Genes for Blastocyst Implantation Using PCR Substration

目的:筛选新的小鼠胚泡着床相关基因。方法:对孕4.5 d小鼠着床和非着床位点的子宫内膜组织进行PCR差减。获得了2个新的在小鼠胚泡着床位点高表达的EST(EST8和EST81)。结果:EST8在孕4.5 d小鼠着床位点、肝脏中表达较高,在非着床位点和卵巢中亦有微量表达。EST81主要在孕4.5 d小鼠子宫着床组织和卵巢中表达,其它各种组织中也有微量表达。用PCR方法获得了相应的全长。cDNA,长度分别为1665bp和1364bp。结论:用差减筛选获得的两种cDNA是孕小鼠着床相关基因,其功能有待进一步研究。

Objective:To identify genes that may be responsible for embryo implantation. Methods:PCR substra-tion was applied in implantation and inters-plantation sites on day 4. 5 of pregnancy in the mouse. Two novel ESTs, EST8 and EST81,were identified, their expression in tissues was analyzed by Northern blotting, and their full length cDNAs were synthesized by PCR. Results: Two novel ESTs (EST8 and EST81) , strongly expressed in implantation site in the mouse. EST8 was expressed abundantly in implantation site on day 4. 5 of pregnancy in the implantation and liver, expressed at low level in inter-plantation site and ovary. EST 81 was present predominantly in implantation site on day 4. 5 of pregnancy in the implantation, at low level in all other tissues. Their complete cDNAs of 1 665 bp and 1 364 bp respectively were synthesized by PCR. Conclusion:The two full length cDNAs were responsible for embryo implantation, and their functions remain to be further studied.