人胃癌裸鼠原位移植和转移模型的建立及两种方法比较

Establishment of Two-orthotopic Implant-metastatic Models of Human Stomach Cancer in Nude Mice

目的:建立人胃癌裸小鼠原位移植和转移模型,并比较两种不同方法的结果。方法:将肿瘤组织块和肿瘤细胞悬液种植于裸小鼠胃壁形成原位移植瘤,观察和比较了两种方法所建立模型肿瘤生长状况、移植成功率和自发转移的发生率。结果:胃癌组织块种植的原位成瘤率达100％,淋巴结广泛转移率90％,肝转移发生率75％。细胞悬液种植的原位成瘤时间较组织块法延迟2周,成瘤率为67％,淋巴结广泛转移率为50％,肝转移发生率仅为30％。结论:两种原位移植和转移模型均具有人胃癌自然生长过程的特点,以肿瘤组织块原位模型为优,该模型的建立可为人胃癌转移机制和抗转移实验治疗的研究提供一种有价值的工具。

Objective: To develop two methods of orthotopic implant-metastatic model of human gastric cancer in nude mice and to compare both methods of the two models. Methods: Intact tumor tissue or tumor cell suspension was orthotopically implanted into the nude mouse stomach to develop implant-tumors. The tumor growth characteristics, tumor-take rates and metastatic rates of the two models were analyzed and compared. Results: Tumor-take rate was 100% in the model implanted with intact tumor tissue. In this model, the metastases of lymph nodes and livers were 90% and 75% respectively. In the model implanted with tumor cell suspension, the local tumor growth was slower, tumor-take rate was 67%. The metastases of lymph nodes and livers were only 50% and 30% respectively. Conclusion: There is growth characteristics of human stomach cancer in both orthotopic tumor models. The model implanted with intact tumor tissue is better than another. This tumor model might be a useful tool in research about metaslalic mechanisms and treatment of anti-metastasis in human stomach cancer.