三羟异黄酮对人乳腺癌细胞株MDA-MB-435S细胞周期的影响

The Effect of Genistein on the Proliferation and Cell Cycle of Human Breast Carcinoma Cell MDA-MB-435S

目的:观察三羟异黄酮(genistein,Gen)对体外培养的人乳腺癌细胞株MDA-MB-435S细胞存活率、细胞周期和凋亡的影响。方法:用噻唑蓝(MTT)法观察Gen对MDA-MB-435S细胞生长的影响;流式细胞仪观察Gen处理人乳腺癌细胞后细胞周期改变的剂量效应和时间效应关系;吖啶橙/溴乙锭染色法(acridine orange/ethidium bromide staining)在荧光显微镜下观察Gen对MDA-MB-435S细胞的凋亡作用。结果:随剂量增大和作用时间延长,Gen对MDA-MB-435S细胞增殖的抑制作用逐渐增强。同时可以阻滞细胞周期于G2-M期,而且随剂量的增大,作用时间的延长,阻滞作用也增强。吖啶橙染色法于荧光显微镜下可见,随Gen剂量增大,细胞凋亡也逐渐明显。结论:Gen可抑制人乳腺癌细胞的增殖,其作用机制可能包括诱导细胞凋亡和G2-M期阻滞。

Objective: To investigate the effect of genistein on the survival rate, cell cycle, and apoptosis of breast carcinoma cell MDA-MB-435S cultured in vitro. Methods: The effects of genistein on the growth of breast carcinoma cells were detected by MTT assay; the relation between the alteration of cell cycle of MDA-MB-435S and different dosage and duration of genistein was observed by flow cytometry, and the action of genistein on apoptosis of MDA-MB-435S cells was observed by fluorescent microscope with acridium orange/ethidium bromide staning. Results: The more inhibitory action of genistein was on the proliferation of MDA-MB-435S cell line, the more dosage and duration of genistein was. Furthermore, genistein could block the cell cycle from G2 to M stage, and the blockage was closed to the dosage and duration of genistein. The cell apoptosis induced by genistein also depended on the dosage. Conclusion: Genstein may inhibite the proliferation of human breast carcinomar cell MDA-MB-435S line in vitro, and genstein may play a role in apoptosis and blocking cell cycle of MDA-MB-435S cell line cultured in vitro.