雷公藤内酯醇对哮喘豚鼠内皮素-1水平影响的动态观察

Effects of Triptolide on the Dynamic Alteration of Endothelin-1 in Plasma and Lung of Asthmatic Model in Guinea-pigs

目的:观察哮喘豚鼠血浆与肺组织内皮素-1(ET-1)、肺组织浸润炎性细胞数的变化,以及雷公藤内酯醇(TP)干预的影响。方法:78只豚鼠随机分为对照组(C)、哮喘组(A)和干预组(T),放射免疫法测定血浆和肺组织ET-1,并对肺组织炎性细胞计数。结果:①0 h外各哮喘组和2、4、8 h干预组肺组织ET-1均显著高于对照组,并均于4 h达高峰。②8 h哮喘组血浆ET-1显著高于对照组。③4、12、24 h哮喘组肺组织嗜酸性粒细胞(EOS)分别显著高于同时段干预组;0 h哮喘组及0、24 h干预组外各组均显著高于对照组。④肺组织ET-1与EOS比较呈正相关(r=O.834,P<0.001)。结论:哮喘时肺组织ET-1升高,且与肺组织EOS数正相关。TP可抑制ET-1的合成、分泌及炎性细胞浸润。

Objective: To investigate the effects of Triptolide(TP) on the dynamic alteration of endothelin-1(ET-1) in plasma and lung of asthmatic model in guinea-pigs. Methods: Seventy-eight male guinea-pigs were randomly divided into 3 groups: control group (C), asthmatic guinea-pig group (A) and asthmatic guinea-pig group intervened (T) with TP. Asthmatic model was prepared in both later groups, ET-1 in plasma and lung of asthmatic guinea-pigs was measured by radioimmunoassay. Results:① ET-1 in lung tissues of all A subgroups except 0 h and 2, 4 and 8 h T subgroups was higher than that of C group. The peak was at 4 h in both groups. ② There was no difference between each subgroup and C group of the plasma ET-1, except 8h asthmatic guinea-pig subgroup that was significantly higher than all the other subgroups and C group.③ Eosinophils in lungs of 4, 12 and 24 h's A subgroups was significantly higher than that of the same time subgroups of T group. ④ And ET-1 in lungs was positively correlated with Eos in lung tissue ( r = 0. 834, P<0. 001). Conclusion: ET-1 in lung tissue is elevated in asthma, and it is positively correlated with Eos in lungs. TP may inhibit the synthesis of ET-1 and infiltration of inflammatory cells.