醒脑静注射液对脑缺血-再灌注损伤家兔血清白介素-8的影响

Effect of Xingnaojing injection on serum interleukin-8 in rabbits following cerebral ischemia-reperfusion injury

目的 :观察醒脑静注射液 (XNJI)对脑缺血 再灌注损伤 (CIRI)家兔血清白介素 8(IL 8)水平的影响 ,并探讨其脑保护机制。方法 :2 8只家兔随机分为假手术对照组 (C组 )、缺血再灌注组 (I R组 )和醒脑静注射液组 (XNJI组 ) ,分别在缺血前、缺血 3 0min、再灌注 3 0min、60min、12 0min时取静脉血 ,测定血清IL 8浓度 ,实验结束后取脑组织观察脑超微结构的变化。结果 :脑缺血 再灌注期间 ,I R组不同时点血清IL 8水平明显高于XNJI组 ,缺血 3 0min为 (0 .62± 0 .18)ng·L-1及 (0 .43± 0 .0 8)ng·L-1,P <0 .0 5 ;再灌注 3 0min ,为血清IL 8水平 (0 .73± 0 .19)ng·L-1及 (0 .45± 0 .0 9)ng·L-1;再灌注 60min ,血清IL 8水平为 (0 .87± 0 .2 9)ng·L-1及 (0 .47± 0 .0 6)ng·L-1;再灌注 12 0min血清IL 8水平为 (1.0 3± 0 .43 )ng·L-1及 (0 .44± 0 .0 7)ng·L-1,再灌注各时点组均P <0 .0 1,超微结构发生异常改变 ;XNJI组不同时点血清IL 8浓度显著低于I R组 (XNJI组缺血 3 0min为(0 .48± 0 .0 7)ng·L-1,P <0 .0 5 ;再灌注 3 0min为 (0 .5 0± 0 .0 8)ng·L-1;再灌注 60min为 (0 .5 5± 0 .14)ng·L-1;再灌注 12 0min为 (0 .5 9± 0 .17)ng·L-1,再灌注各时点组P <0 .0 1) ,其超微结构异常改变较I R组?

Objective:To determine the inhibitory effect of xingnaojing injection(XNJI) on the level of serum interleukin 8(IL 8) following cerebral ischemia and reperfusion injury(CIRI) in rabbits.Methods:28 rabbits were randomly divided into three groups:control group(group C),ischemia reperfusion group(group I R)and XNJI group(group XNJI). Blood samples were obtained through vein,to measure the serum concentration of IL 8 25 min before ischemia,ischemia 30 min,30 min,60 min and 120 min after reperfusion. The ultrastructure changes of the cerebral tissue were observed.Results:Serum IL 8 levels at different time point in group I R were significantly higher than those of the group C(P<0.05 and P<0.01). The abnormal morphological changes of cerebral cells under electron microscope were more obvious in the group I R. Serum IL 8 levels at different time point in the group XNJI were remarkably lower than those of the group I R(P<0.05 and P<0.01);the abnormal morphyological changes of cerebral cells were all alleviated markedly after treatment of XNJI.Conclusion:XNJI can effectively decrease serum IL 8,protect the brain from ischemia and reperfusion injury.